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anti her3 antibody  (R&D Systems)


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    R&D Systems anti her3 antibody
    Anti Her3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+her3+antibody/Human+ErbB3%2FHer3+Biotinylated+Antibody/us11754557-643-29-32
    Average 90 stars, based on 1 article reviews
    anti her3 antibody - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Blood‐based markers of efficacy and resistance to cetuximab treatment in metastatic colorectal cancer: results from CALGB 80203 (Alliance)
    Article Snippet: For HER3, ELISA plates were coated overnight with 4 μ g/mL HER3 capture antibody (MAB3481; R&D Systems, Minneapolis, MN). .. After sample incubation, HER3 was detected using 1 μ g/mL biotinylated HER3 antibody (MN BAF234; R&D Systems) and 5 μ g/mL streptavidin‐conjugated SulfoTag (R32AD‐5; Meso Scale Discovery). .. For CD73, ELISA plates were coated overnight with 3.3 μ g/mL CD73 capture antibody (550256; BD Biosciences, San Jose, CA).



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    Image Search Results


    Protein expression and frequency distribution in primary and metastatic lung cancer tissue. EGFR protein immunohistochemistry ( A ) and HER3 protein immunohistochemistry ( B ) was performed on tissue microarray from primary NSCLC tumors and brain metastases. Representative strong (left), intermediate (center) and negative (right) staining are shown in 200x magnification (Zeiss Axiovision).

    Journal: Scientific Reports

    Article Title: EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients

    doi: 10.1038/s41598-019-43678-6

    Figure Lengend Snippet: Protein expression and frequency distribution in primary and metastatic lung cancer tissue. EGFR protein immunohistochemistry ( A ) and HER3 protein immunohistochemistry ( B ) was performed on tissue microarray from primary NSCLC tumors and brain metastases. Representative strong (left), intermediate (center) and negative (right) staining are shown in 200x magnification (Zeiss Axiovision).

    Article Snippet: For HER3 we tested two different antibodies; the biotinylated human anti-HER3 antibody (clone REA508; Miltenyi Biotec, Bergisch Gladbach, Germany) and the biotinylated human anti-HER-3 antibody (clone 1B4C3; BioLegend, San Diego, California).

    Techniques: Expressing, Immunohistochemistry, Microarray, Staining

    EGFR ( A ) and HER3 ( B ) expression distribution in primary lung, lymph node and brain metastatic tissue and association between HER3 protein expression in primary and clinicopathological parameters ( C ). HER3 is more frequently expressed in brain metastasis of oligo-brain metastatic patients (p = 0.028) compared to patients with other metastatic sites. HER3 expression in primary NSCLC tumors is significantly associated with a decreased time to metastatic progression (p = 0.006; log-rank test) ( D ) and decreased relapse-free survival time (p = 0.013; log-rank test) ( E ).

    Journal: Scientific Reports

    Article Title: EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients

    doi: 10.1038/s41598-019-43678-6

    Figure Lengend Snippet: EGFR ( A ) and HER3 ( B ) expression distribution in primary lung, lymph node and brain metastatic tissue and association between HER3 protein expression in primary and clinicopathological parameters ( C ). HER3 is more frequently expressed in brain metastasis of oligo-brain metastatic patients (p = 0.028) compared to patients with other metastatic sites. HER3 expression in primary NSCLC tumors is significantly associated with a decreased time to metastatic progression (p = 0.006; log-rank test) ( D ) and decreased relapse-free survival time (p = 0.013; log-rank test) ( E ).

    Article Snippet: For HER3 we tested two different antibodies; the biotinylated human anti-HER3 antibody (clone REA508; Miltenyi Biotec, Bergisch Gladbach, Germany) and the biotinylated human anti-HER-3 antibody (clone 1B4C3; BioLegend, San Diego, California).

    Techniques: Expressing

    CTC positivity rates in NSCLC patients (n = 45). ( A ) Significantly higher detection rates when combining the methods in comparison to either single use CellSearch (p = 0.0023) or magnetic cell separation with EGFR/HER3 (p = 0.0109). ( B ) CTC enumeration detected by CellSearch and MACS.

    Journal: Scientific Reports

    Article Title: EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients

    doi: 10.1038/s41598-019-43678-6

    Figure Lengend Snippet: CTC positivity rates in NSCLC patients (n = 45). ( A ) Significantly higher detection rates when combining the methods in comparison to either single use CellSearch (p = 0.0023) or magnetic cell separation with EGFR/HER3 (p = 0.0109). ( B ) CTC enumeration detected by CellSearch and MACS.

    Article Snippet: For HER3 we tested two different antibodies; the biotinylated human anti-HER3 antibody (clone REA508; Miltenyi Biotec, Bergisch Gladbach, Germany) and the biotinylated human anti-HER-3 antibody (clone 1B4C3; BioLegend, San Diego, California).

    Techniques: Comparison, Magnetic Cell Separation

    Representative images of two Circulating Tumor Cell cluster (DAPI + /CD45 − /CK + ) isolated via magnetic cell separation with HER3-protein antibody in one NSCLC patient. Heterogeneous MET expression was found within the cluster as well as CD45-positive leukocytes.

    Journal: Scientific Reports

    Article Title: EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients

    doi: 10.1038/s41598-019-43678-6

    Figure Lengend Snippet: Representative images of two Circulating Tumor Cell cluster (DAPI + /CD45 − /CK + ) isolated via magnetic cell separation with HER3-protein antibody in one NSCLC patient. Heterogeneous MET expression was found within the cluster as well as CD45-positive leukocytes.

    Article Snippet: For HER3 we tested two different antibodies; the biotinylated human anti-HER3 antibody (clone REA508; Miltenyi Biotec, Bergisch Gladbach, Germany) and the biotinylated human anti-HER-3 antibody (clone 1B4C3; BioLegend, San Diego, California).

    Techniques: Isolation, Magnetic Cell Separation, Expressing